Thursday, February 11, 2010

Who designed the cuticle prep for Drosophila?

This might point you in the right direction:





Wieschaus, E. and C. N眉sslein-Volhard. 1986. Looking at embryos. In Drosophila: A practical approach (ed. D.B. Roberts), pp. 199-286. RL Press, Oxford, UK.





This is the process used in this article:


http://www.genesdev.org/cgi/content/full鈥?/a>


';Cuticle preparation was performed as descibed (Wieschaus and N眉sslein-Volhard 1986). In brief, the embryos were collected and dechorionated as described above and fixed in glycerol:acetic acid (1:1) at 60掳C for 1 hour. The cuticles were visualized after incubation in Hoyers:lactate overnight at 60掳C.';





Best of luck -Who designed the cuticle prep for Drosophila?
me

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